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Updated: Jul 17, 2026

Autofluorescence Imaging to Evaluate Red Algae Physiology
Published on: February 17, 2023
Microspectroscopy of the photosynthetic compartment of algae
Valtere Evangelista1, Anna Maria Frassanito, Vincenzo Passarelli
1Istituto di Biofisica, Area della Ricerca CNR, Pisa, Italy.
Abstract:
We performed microspectroscopic evaluation of the pigment composition of the photosynthetic compartments of algae belonging to different taxonomic divisions and higher plants. The feasibility of microspectroscopy for discriminating among species and/or phylogenetic groups was tested on laboratory cultures. Gaussian bands decompositions and a fitting algorithm, together with fourth-derivative transformation of absorbance spectra, provided a reliable discrimination among chlorophylls a, b and c, phycobiliproteins and carotenoids. Comparative analysis of absorption spectra highlighted the evolutionary grouping of the algae into three main lineages in accordance with the most recent endosymbiotic theories.
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