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Related Experiment Videos

Multidrug resistance activity in human lymphocytes.

J S Coon1, Y Z Wang, S D Bines

  • 1Department of Pathology, Rush Presbyterian St. Luke's Medical Center, Chicago, Illinois.

Human Immunology
|October 1, 1991
PubMed
Summary

Normal lymphocytes exhibit multidrug resistance (MDR)-like activity, effluxing the dye rhodamine 123. This efflux varies among lymphocyte subsets and is modulated by MDR-reversing agents, suggesting a role in lymphocyte function.

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Area of Science:

  • Cell Biology
  • Immunology
  • Molecular Biology

Background:

  • The multidrug resistance gene (mdr1) belongs to the ATP binding cassette (ABC) transporter superfamily.
  • MDR1 expression correlates with cellular efflux of chemotherapeutic agents and lipophilic compounds like rhodamine 123.
  • Previous studies suggest normal lymphocytes possess MDR-like activity.

Purpose of the Study:

  • To investigate the presence and characteristics of MDR-like activity in human lymphocyte subsets.
  • To determine if rhodamine 123 efflux is heterogeneous among lymphocytes.
  • To assess the modulation of rhodamine 123 transport by MDR reversing agents.

Main Methods:

  • Two-color flow cytometric analysis was employed.
  • Rhodamine 123 efflux and accumulation in lymphocytes were measured.

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  • The effects of verapamil and Solutol HS 15 on rhodamine 123 transport were evaluated.

    • Main Results:

    • Rhodamine 123 efflux was heterogeneous across human lymphocyte subsets: CD8 > CD4 > CD20.
    • Lymphocyte rhodamine 123 efflux and accumulation were sensitive to verapamil and Solutol HS 15.
    • These findings indicate MDR-like transport activity in normal lymphocytes.

    Conclusions:

    • Normal lymphocytes express an MDR-like transport system.
    • This system exhibits differential activity among CD4, CD8, and CD20 subsets.
    • The MDR-like system may play a role in the transport of molecules crucial for lymphocyte function.