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[Determination of ginsenoside rd in human plasma by LC/MS/MS]
Yuan-hui Deng1, Yi Feng, Xing Zeng
1Department of Clinical Pharmacology, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, China.
Objective:
To develop a HPLC/MS/MS method for the determination of ginsenoside Rd in human plasma.
Methods:
Plasma samples were pretreated by solid phase extraction (SPE). Ginsenoside Rd (m/z 964.6-->m/z 767.5) and gentiopicrin (m/z 374.1-->195.1) was detected by the positive electrospray ionization (ES+I)-MS method under multiple reaction monitoring (MRM) mode.
Results:
The calibration curve of ginsenoside Rd in plasma was linear over the range of 3.00-5000.00 ng/ml. The limit of quantitation was 3.00 ng/ml. The relative recovery was 81.01-83.39%. The within-day and between-day RSDs were less than 15%. CONCLUSION This method is accurate, sensitive, and specific. It is suitable for the measurement of plasma ginsenoside Rd concentration.
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