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Related Experiment Video

Updated: May 23, 2026

Using Caco-2 Cells to Study Lipid Transport by the Intestine
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Using Caco-2 Cells to Study Lipid Transport by the Intestine

Published on: August 20, 2015

[The evaluation criterions about Caco-2 cell model].

Yan Ma1, Pei-bo Li, Wei-wei Su

  • 1School of Life Science, Sun Yat-sen University, Guangzhou 510275, China.

Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials
|January 11, 2007
PubMed
Summary
This summary is machine-generated.

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This study established a reliable Caco-2 cell model for drug absorption research. The model demonstrated superior integrity, permeability, and alkaline phosphatase expression, validating its use in oral drug absorption studies.

Area of Science:

  • Pharmacokinetics and Drug Metabolism
  • Cell Biology and Tissue Engineering

Context:

  • The Caco-2 cell line is a widely used in vitro model for predicting intestinal drug absorption.
  • Standardization and validation of this model are crucial for reliable preclinical drug assessment.

Purpose:

  • To establish and rigorously evaluate a Caco-2 cell model for its suitability in drug absorption studies.
  • To assess key parameters including morphology, transepithelial electrical resistance (TEER), alkaline phosphatase activity, and propranolol transport.

Summary:

  • The Caco-2 cell model was successfully established and characterized.
  • Morphological features, TEER, alkaline phosphatase expression, and propranolol transport were evaluated and found to be consistent with established reference values.
  • The model exhibited superior integrity, permeability, and alkaline phosphatase expression.

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Published on: January 30, 2018

The Caco-2 Cell Bioassay for Measurement of Food Iron Bioavailability
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Related Experiment Videos

Last Updated: May 23, 2026

Using Caco-2 Cells to Study Lipid Transport by the Intestine
07:00

Using Caco-2 Cells to Study Lipid Transport by the Intestine

Published on: August 20, 2015

A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC
11:40

A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC

Published on: January 30, 2018

The Caco-2 Cell Bioassay for Measurement of Food Iron Bioavailability
06:34

The Caco-2 Cell Bioassay for Measurement of Food Iron Bioavailability

Published on: April 28, 2022

Impact:

  • Provides a validated in vitro model for studying oral drug absorption mechanisms.
  • Enhances the reliability of preclinical drug screening and development.
  • Contributes to a better understanding of drug permeability and bioavailability in the gastrointestinal tract.