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[Study on purification of Radix Paeoniae Alba by HPD100 macroporous resin]
1Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Objective:
To set up the method for purification of paeoniflorin by HPD100 macroporous resin.
Methods:
With the adsorption ration and desorption rate of paeoniflorin as the evaluating quotas, the single factor test, orhogonal experimental designs and variance analysis were applied to optimize the manipulation parameters of macrporous resin.
Results:
The HPD100 had the best adsorbive and separative properties to paeoniflorin. The applicable technology was as follows: the sample concentration was 0.5 g/ml, the maximum capacity for medicinal materials was 10 g/g, the current velocit was 2 BV/h, the eluting reagent was 30% ethanol (4 times the volume of bottle).
Conclusion:
HPD100 resin can be used in purify of Radix Paeoniae Alba successfully. The purity of paeoniflorin was above 30%.
