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Updated: Jul 17, 2026

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
Published on: October 15, 2018
MPAI (mass probes aided ionization) method for total analysis of biomolecules by mass spectrometry
Aki Honda1, Shinichiro Hayashi, Hiroki Hifumi
1Department of Applied Chemistry, Keio University, Yokohama, Kanagawa, Japan.
Abstract:
We have designed and synthesized various mass probes, which enable us to effectively ionize various molecules to be detected with mass spectrometry. We call the ionization method using mass probes the "MPAI (mass probes aided ionization)" method. We aim at the sensitive detection of various biological molecules, and also the detection of bio-molecules by a single mass spectrometry serially without changing the mechanical settings. Here, we review mass probes for small molecules with various functional groups and mass probes for proteins. Further, we introduce newly developed mass probes for proteins for highly sensitive detection.
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