Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
ER Retrieval Pathway01:45

ER Retrieval Pathway

In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Catenins01:23

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
Selectins01:25

Selectins

Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A New Deep Learning Network for Mitigating Limited-view and Under-sampling Artifacts in Ring-shaped Photoacoustic Tomography.

Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society·2020
Same author

Effects of osmotic pressure and pH on citric acid and erythritol production from waste cooking oil by <i>Yarrowia lipolytica</i>.

Engineering in life sciences·2020
Same author

Facile formulation of a long-wavelength cyanine for optical imaging in the second near-infrared window.

Biomaterials science·2020
Same author

Obesity induces preadipocyte CD36 expression promoting inflammation via the disruption of lysosomal calcium homeostasis and lysosome function.

EBioMedicine·2020
Same author

Glucocorticoid therapy delays the clearance of SARS-CoV-2 RNA in an asymptomatic COVID-19 patient.

Journal of medical virology·2020
Same author

Innovation Strategy Selection Facilitates High-Performance Flexible Piezoelectric Sensors.

Sensors (Basel, Switzerland)·2020
Same journal

A Comprehensive Review of Melatonin as Multi-Pathway Neuroprotectant Against Methamphetamine-Induced Programmed Cell Death: Discovery of the Circadian-Ferroptosis Axis.

Neuro-Signals·2026
Same journal

The Impact of Vitamin D Deficiency on Cognitive and Neuromuscular Functions in Elderly Patients.

Neuro-Signals·2026
Same journal

Tracing the Evolution and Recombination Events of Neurotropic Arenaviridae Viruses: a Bioinformatics Approach.

Neuro-Signals·2025
Same journal

Peripheral Inflammation as a Biomarker of Disease Activity in Relapsing-Remitting MS.

Neuro-Signals·2025
Same journal

Aberrant Hippocampal Neuroregenerative Plasticity in Schizophrenia: Reactive Neuroblastosis as a Possible Pathocellular Mechanism of Hallucination.

Neuro-Signals·2024
Same journal

A Narrative Review - Therapy Options and Therapy Failure in Retinoblastoma.

Neuro-Signals·2022
See all related articles

Related Experiment Video

Updated: Jun 21, 2026

Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells
07:38

Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells

Published on: September 11, 2013

Structure and function of PICK1.

Junyu Xu1, Jun Xia

  • 1Department of Biochemistry, The Hong Kong University of Science and Technology, Kowloon, Hong Kong, SAR, China.

Neuro-Signals
|January 12, 2007
PubMed
Summary
This summary is machine-generated.

Protein interacting with membranes (PICK1) binds both proteins and lipids. This dual binding regulates protein localization and trafficking, crucial for cellular functions in the brain and other tissues.

More Related Videos

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
10:50

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Published on: March 14, 2019

Related Experiment Videos

Last Updated: Jun 21, 2026

Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells
07:38

Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells

Published on: September 11, 2013

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
10:50

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Published on: March 14, 2019

Area of Science:

  • Molecular and Cellular Biology
  • Neuroscience
  • Biochemistry

Background:

  • PICK1 is a conserved peripheral membrane protein found in various tissues, notably the brain and testes.
  • It localizes to the perinuclear region and neuronal synapses, indicating specific cellular roles.

Purpose of the Study:

  • To elucidate the structural and functional mechanisms of PICK1 in regulating membrane protein localization.
  • To understand how PICK1's unique domains mediate interactions with both proteins and lipids.

Main Methods:

  • Analysis of PICK1's domain structure, including its PDZ and BAR domains.
  • Investigation of PICK1's binding interactions with membrane proteins and phosphoinositides.
  • Assessment of PICK1's role in regulating the subcellular localization and surface expression of its binding partners.

Main Results:

  • PICK1's PDZ domain binds numerous membrane proteins, particularly those with C-terminal type II PDZ-binding motifs.
  • PICK1's BAR domain interacts with lipid molecules, primarily phosphoinositides, with binding modulated by other PICK1 regions.
  • PICK1 binding to lipids is essential for its regulatory function on protein localization and surface expression.

Conclusions:

  • PICK1 acts as a molecular bridge, coupling membrane proteins to cellular trafficking machinery via its dual-domain interactions.
  • The structure of PICK1, with its PDZ and BAR domains, is key to its function in protein localization and trafficking.
  • PICK1 plays a significant role in cellular processes by mediating protein-lipid and protein-protein interactions.