Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Successful Treatment of Tubulointerstitial Nephritis With Uveitis Syndrome Using Adalimumab.

Pediatrics international : official journal of the Japan Pediatric Society·2026
Same author

Pericaval Fat Collection in a Patient With Systemic Juvenile Idiopathic Arthritis.

International journal of rheumatic diseases·2026
Same author

Cutaneous Magnetic Stimulation for Chronic Pain in Children: A Case Report.

Pediatrics international : official journal of the Japan Pediatric Society·2026
Same author

Kawasaki disease complicated by periaortitis.

Clinical rheumatology·2026
Same author

Survival by Detection Pathway in Patients With Lung Cancer-A Nationwide Descriptive Epidemiological Study in Japan.

International journal of cancer·2026
Same author

Biallelic variants in FAT3 cause axonal neuropathy with multisystem neurodevelopmental features.

Genetics in medicine : official journal of the American College of Medical Genetics·2026

Related Experiment Video

Updated: Jul 17, 2026

Live-imaging of the Drosophila Pupal Eye
09:54

Live-imaging of the Drosophila Pupal Eye

Published on: January 12, 2015

Complex interference in the eye developmental pathway by Drosophila NF-YA.

Yasuhide Yoshioka1, Osamu Suyari, Mikihiro Yamada

  • 1Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, Japan.

Genesis (New York, N.Y. : 2000)
|January 12, 2007
PubMed
Summary

The CCAAT motif-binding factor NF-YA is crucial for Drosophila development. Overexpression disrupts eye development, indicating its role in eye disc specification.

More Related Videos

Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis
07:49

Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis

Published on: August 27, 2011

Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging
16:47

Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging

Published on: March 15, 2010

Related Experiment Videos

Last Updated: Jul 17, 2026

Live-imaging of the Drosophila Pupal Eye
09:54

Live-imaging of the Drosophila Pupal Eye

Published on: January 12, 2015

Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis
07:49

Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis

Published on: August 27, 2011

Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging
16:47

Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging

Published on: March 15, 2010

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • The NF-Y complex, composed of NF-YA, NF-YB, and NF-YC subunits, binds to CCAAT motifs and is essential for DNA binding.
  • NF-YA possesses a DNA-binding domain in its C-terminal region.
  • NF-Y plays roles in various gene regulatory pathways.

Purpose of the Study:

  • To investigate the function of NF-YA during Drosophila development.
  • To determine the effects of NF-YA overexpression and knockdown on developmental processes.
  • To elucidate the role of NF-YA in eye development and specification.

Main Methods:

  • Generation of transgenic Drosophila lines expressing UAS-HA-dNF-YA or UAS-dNF-YAIR.
  • Utilizing GAL4 drivers for spatial and temporal control of gene expression (overexpression and knockdown).
  • Phenotypic analysis of developmental stages, including lethality and specific morphological defects like the headless phenotype.

Main Results:

  • Overexpression or knockdown of dNF-YA using various GAL4 drivers led to lethality at different developmental stages.
  • Expression of dNF-YA with eyeless-GAL4 primarily caused lethality and a headless phenotype in pharate-adults.
  • Reduction of the eyeless gene dose exacerbated the dNF-YA phenotype, while reduction of Distal-less suppressed it.
  • Interactions with Notch mutants did not significantly alter the dNF-YA phenotype.

Conclusions:

  • dNF-YA is involved in critical gene regulatory pathways during Drosophila development.
  • dNF-YA plays a significant role in eye disc specification.
  • dNF-YA does not appear to be essential for eye disc growth, but rather for its proper formation.