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Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
Published on: January 21, 2016
Paenibacillus terrigena sp. nov., isolated from soil
1Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan.
Abstract:
A novel Gram-positive bacterium, strain A35(T), was isolated from coastal soil at Chiba, Japan, and was identified as a member of the genus Paenibacillus on the basis of phenotypic and phylogenetic analyses. The bacterium was found to be a facultatively anaerobic and endospore-forming rod. The predominant menaquinone was MK-7, the major cellular fatty acid was anteiso-C(15 : 0) and the DNA G+C content was 48.1 mol%. The levels of 16S rRNA gene sequence similarity between strain A35(T) and Paenibacillus species with validly published names were less than 94 %. Strain A35(T) was clearly distinguishable from reference species for the genus Paenibacillus. Therefore, on the basis of these data, a novel species of the genus Paenibacillus, Paenibacillus terrigena sp. nov., is proposed. The type strain is A35(T) (=IAM 15291(T)=CCTCC AB206026(T)).
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