Related Experiment Videos
Gas-related impact of pneumoperitoneum on systemic wound healing
R Rosch1, K Junge, M Binnebösel
1Department of Surgery, University Hospital Aachen, Aachen, Germany. r.rosch@chir.rwth-aachen.de
Introduction:
The aim of the present study was to investigate the gas-dependent effects of pneumoperitoneum on wound healing distant from the abdomen.
Materials And Methods:
Dorsal skin incisions were performed in 54 male Sprague-Dawley rats. A CO(2) or a helium pneumoperitoneum of 3 mmHg was maintained before, with an overall duration of 30 min (each n = 18). Rats in the control group received laparotomy only (n = 18). Animals were killed after 5 and 10 days. The infiltration of macrophages (CD 68), expression of matrix metalloproteinase 13 (MMP-13) and collagen I/III ratios were analysed in the dorsal skin wounds.
Results:
Both after 5 and 10 days, infiltration of macrophages and expression of MMP-13 were greatest after helium pneumoperitoneum. After 5 days, collagen I/III ratio was significantly increased in the helium group. After 10 days, collagen I/III ratio was lowest in the CO(2) group.
Conclusion:
Our results suggest beneficial effects on systemic wound healing for helium pneumoperitoneum as compared to CO(2).
Related Concept Videos
Pneumothorax II: Pathophysiology
Healing II: Complications
Pneumothorax-II
Clinical Manifestations:
Atelectasis II: Pathophysiology
Pneumothorax-I
Pneumothorax can be even further classified as spontaneous, traumatic, and tension pneumothorax.
Peptic Ulcer Disease V: Surgical Management and Nursing Care
Surgical Interventions for Peptic Ulcer Disease