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Updated: Jul 17, 2026
Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)
Published on: August 18, 2017
A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the estimation of
Jignesh Bhatt1, Sadhana Singh, Gunta Subbaiah
1Department of Chemistry, BNPG College, Mohanlal Sukhadia University, Udaipur, Rajasthan State, India. jigu_baroda@rediffmail.com
Abstract:
A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the estimation of amlodipine in human plasma. Amlodipine was extracted from human plasma by using a solid-phase extraction technique. Imipramine was used as the internal standard. A Hypersil BDS C18 column provided chromatographic separation of analytes followed by detection with mass spectrometry. The method involves a rapid solid-phase extraction from plasma, simple isocratic chromatography conditions and mass spectrometric detection that enables detection at sub-nanogram levels. The proposed method has been validated for a linear range of 0.1-10.0 ng/mL with correlation coefficient >or=0.9990. The intrarun and interrun precision and accuracy were within 10.0%. The overall recovery for amlodipine was 63.67%. Total run time was 3.2 min only.
