A novel epitope expressed on the surface of developing and mature astrocytes

Plasmalemmal fractions from cultured astrocytes have been used as the immunogen in generating a monoclonal antibody, termed 8C10, which binds to the surface of cultured astrocytes of the rat. 8C10 immunoreactivity is present on the membrane surface of cultured type 1 astrocytes, type 2 astrocytes, oligodendrocytes, meningeal cells, and 02A progenitor cells, and it persists after aldehyde fixation. The antibody also stains aldehyde-fixed central nervous system, in a pattern which suggests that the plasma membranes of fine astrocytic processes in adult neuropil express the epitope. Astrocytic perikarya and processes in white matter are also stained, but there is no immunoreactivity present in neuronal processes or perikarya. Astrocytic processes in developing cerebellar cortex are stained at postnatal ages when some of these processes are guiding the migration of neuronal perikarya.