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Updated: Jul 17, 2026

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
Published on: August 31, 2021
Dual-trap technique for reduction of low-frequency noise in force measuring optical tweezers
Markus Klein1, Magnus Andersson, Ove Axner
1Department of Physics, Umeå University, Sweden.
Abstract:
High-resolution long-time force measurements by optical tweezers are often limited by low-frequency (1/f) noise. A dual-trap technique is presented that can reduce such noise in the force signal. It incorporates a second trap (a reference trap) that probes the noise in the system and it is based upon the assumption that the low-frequency parts of the noise from the two traps are correlated. A subtraction of the low-frequency signal from the reference trap from the signal from the force measuring trap will therefore yield a net signal that is significantly less influenced by noise. It is shown that this dual-trap technique can reduce the noise in the force signal up to 60% depending on detection bandwidth.

