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Updated: Jul 17, 2026
A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
Published on: April 1, 2019
[Detection of short tandem repeat (STR) polymorphisms by microchip electrophoresis for individual identification of
Akihiro Yamaguchi1, Kaori Shimizu, Takashi Mishima
1Japan Food Research Laboratories, Chitose, Bunkyo 2-3, Chitose-shi, Hokkaido 066-0052, Japan.
Abstract:
A simple and rapid detection of short tandem repeat (STR) markers was studied as a screening test for individual identification of cattle. DNAs were extracted from eight commercial beef samples by a proteinase K-boil method followed by purification with 2-propanol precipitation. Five STR markers, known to be highly polymorphic, were amplified by PCR and analyzed both by a conventional sequencing analysis (SEQ) and by a proposed microchip electrophoresis (MEP). Every marker revealed high polymorphism, such as 5-9 alleles in SEQ analysis, and 4-6 alleles in MEP analysis. This simple and rapid MEP analysis is expected to be an effective screening tool with use of confirmatory SEQ analysis.
