Characterization of the maize Globulin-2 gene and analysis of two null alleles

The most abundant proteins present in maize (Zea mays L.) embryos are saline-soluble globulins. A Mr 45,000 globulin component, designated GLB2, is encoded by the Glb2 gene. A cDNA clone corresponding to Glb2 was used as radiolabeled probe to examine the expression of Glb2 in developing embryos and other maize tissues. Glb2 transcripts accumulate during embryo development and are not detectable in germinating kernels. Glb2 transcripts are found only in the developing embryo, and not in endosperm, seedling, or unfertilized ears. Analysis of globulin profiles in embryos homozygous for either a previously described null allele, Glb-2-0, or a novel null allele, Glb2-N1, revealed that these embryos lack not only the GLB2 protein but also globulins of lower molecular mass which may represent processed forms of GLB2. Southern blot analysis of DNA from Glb2-0/0 and Glb2-N1/N1 plants in which a Glb2-specific clone is used as probe indicates that the two null alleles are genetically distinct.