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Immunoaffinity purification of human alpha-fetoprotein (AFP) using monoclonal antibodies
1Department of Experimental and Clinical Immunology, Central Institute of Molecular Biology, Berlin-Buch, Germany.
Biomedica Biochimica Acta
|January 1, 1991
Abstract:
A two-step immunoaffinity isolation procedure for human alpha-fetoprotein (AFP) was developed resulting in highly purified AFP at high yield. A monoclonal antibody immunoadsorbent was used in the first step. Elution of AFP was carried out at alkaline pH by a solution of 0.5 mol/l ammonia containing 0.5 mol/l sodium chloride. To remove impurities caused by the first step, an anti-mouse immunoglobulin antibody immunoadsorbent was applied in the second step.