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Immunohistochemical and molecular study of severe cervical dysplasia associated with HPV-83
Valérie Albrecht1, Anne Chevallier, André Bongain
1Laboratoire de Virologie, BP 3079, Hôpital de l'Archet, 151 route de Saint-Antoine de Ginestière, 06200 Nice cedex 3, France.
Background:
Based on epidemiological data, infections with specific types of HPV can be classified as high-risk (HR), low-risk (LR) or intermediate-risk (IR) HPV depending on the risk of progression to cervical cancer.
Case:
A 70-year-old woman consulted for relapse of abnormal cytology with high-grade squamous intraepithelial lesions (HSIL) associated with HPV-83 infection. Histological examination demonstrated high-grade cervical intraepithelial neoplasia (CIN III) with strong and diffuse staining by the P16(INK4a)-specific antibody.
Conclusion:
This patient's intermediate-risk HPV infection (HPV-83) rapidly progressed to severe cervical intraepithelial neoplasia (CIN III) with strong anti-P16(INK4a) immunolabelling. Analysis of the E6 peptide sequence revealed several mutations in one of the two putative zinc finger regions (AA residues 107-135) associated with p53 binding.
