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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jul 17, 2026

High-throughput Protein Expression Generator Using a Microfluidic Platform
09:26

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

One-dimensional microfluidic beads array for multiple mRNAs expression detection.

Jianhui Wen1, Xiaohai Yang, Kemin Wang

  • 1State Key Laboratory of Chemo/Biosensing and Chemometrics, Biomedical Engineering Center, College of Chemistry and Chemical Engineering, Hunan University, Engineering Research Center for Bio-Nanotechnology of Hunan Province, Changsha, PR China.

Biosensors & Bioelectronics
|January 24, 2007
PubMed
Summary

This study introduces a microfluidic beads array for rapid, simultaneous measurement of multiple messenger RNA (mRNA) expressions. This technology shows promise for early cancer diagnostics by analyzing tumor-associated gene expression with high sensitivity.

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Last Updated: Jul 17, 2026

High-throughput Protein Expression Generator Using a Microfluidic Platform
09:26

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Published on: August 23, 2012

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genomics

Background:

  • Accurate measurement of multiple mRNA expressions is crucial for understanding cellular processes and disease states.
  • Existing methods can be time-consuming and require large sample volumes.
  • Development of high-throughput, low-sample-volume techniques is needed for efficient gene expression analysis.

Purpose of the Study:

  • To develop and validate a one-dimensional microfluidic beads array for rapid, simultaneous detection of multiple mRNA targets.
  • To evaluate the expression levels of tumor-associated genes (p53, H-ras, NME1) in nasopharyngeal carcinoma cell lines and normal cells.
  • To assess the response of these gene expressions to 5-fluorouracil (5-Fu) treatment.

Main Methods:

  • Fabrication of a one-dimensional microfluidic channel with immobilized gene-specific DNA-functionalized beads.
  • Simultaneous detection of multiple nucleic acid targets using the microfluidic beads array.
  • Quantification of mRNA expression for p53, H-ras, and NME1 in CNE2 cells and normal nasopharyngeal epithelial cells.
  • Assessment of gene expression changes in response to 5-fluorouracil (5-Fu) treatment.
  • Validation of results using reverse transcriptase-polymerase chain reaction (RT-PCR).

Main Results:

  • The microfluidic beads array successfully performed simultaneous detection of multiple nucleic acid targets.
  • A DNA detection limit of 0.02 nM was achieved, demonstrating high sensitivity.
  • Differential expression of p53, H-ras, and NME1 was observed between CNE2 cancer cells and normal cells.
  • The array effectively monitored the response of these genes to 5-Fu treatment in CNE2 cells.
  • RT-PCR validation confirmed the results obtained from the microfluidic array.

Conclusions:

  • The developed one-dimensional microfluidic beads array offers a rapid and sensitive method for multiplexed mRNA expression analysis.
  • This technology integrates the high throughput of microarrays with the low sample consumption and convenient liquid handling of microfluidics.
  • The platform holds significant potential for applications in early cancer diagnostics and molecular biology research.