Related Experiment Video
Updated: Aug 2, 2026
12:11
ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
Published on: October 23, 2009
[A highly sensitive immunoenzyme analysis using electrochemical detection]
Laboratornoe Delo
|January 1, 1991
Summary
This study introduces a sensitive electrochemical enzyme immunoassay method. It detects blood serum antibodies and antigens at 10(-11)-10(-12) M, potentially aiding asthma and bronchitis treatment monitoring.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Immunology
Context:
- Electrochemical enzyme immunoassays are crucial for detecting biomarkers.
- Improving assay sensitivity is essential for early disease detection and monitoring.
- Current methods may lack the sensitivity for subtle biomarker changes.
Purpose:
- To develop a highly sensitive electrochemical enzyme immunoassay.
- To enhance the detection limit for blood serum antibodies and antigens.
- To validate the method for clinical applications in respiratory diseases.
Summary:
- A novel method enhances electrochemical enzyme immunoassay sensitivity by complexing volatile enzymatic reaction products.
- The complex is subsequently destroyed in a flow-type amperometric detector, enabling ultra-sensitive detection.
- This technique achieves a detection limit of 10(-11)-10(-12) M for antibodies and antigens.
Impact:
- The method successfully measured immunoglobulin E (IgE) levels in patients with asthma and chronic obstructive bronchitis.
- A reduction in IgE levels post-therapy was consistently observed.
- This suggests the assay's potential as a criterion for evaluating treatment efficacy in these conditions.
Related Concept Videos
01:33Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
01:20Immunogold Electron Microscopy
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.