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[A highly sensitive immunoenzyme analysis using electrochemical detection].

R A Sitdykov, D M Ivnitskiĭ, A A Davidian

    Laboratornoe Delo
    |January 1, 1991
    PubMed
    Summary
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    This study introduces a sensitive electrochemical enzyme immunoassay method. It detects blood serum antibodies and antigens at 10(-11)-10(-12) M, potentially aiding asthma and bronchitis treatment monitoring.

    Area of Science:

    • Analytical Chemistry
    • Biochemistry
    • Immunology

    Context:

    • Electrochemical enzyme immunoassays are crucial for detecting biomarkers.
    • Improving assay sensitivity is essential for early disease detection and monitoring.
    • Current methods may lack the sensitivity for subtle biomarker changes.

    Purpose:

    • To develop a highly sensitive electrochemical enzyme immunoassay.
    • To enhance the detection limit for blood serum antibodies and antigens.
    • To validate the method for clinical applications in respiratory diseases.

    Summary:

    • A novel method enhances electrochemical enzyme immunoassay sensitivity by complexing volatile enzymatic reaction products.
    • The complex is subsequently destroyed in a flow-type amperometric detector, enabling ultra-sensitive detection.

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  • This technique achieves a detection limit of 10(-11)-10(-12) M for antibodies and antigens.

    • Impact:

    • The method successfully measured immunoglobulin E (IgE) levels in patients with asthma and chronic obstructive bronchitis.
    • A reduction in IgE levels post-therapy was consistently observed.
    • This suggests the assay's potential as a criterion for evaluating treatment efficacy in these conditions.