Related Experiment Video
Updated: Aug 2, 2026

07:42
Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
Published on: October 13, 2015
[A method of indirect immunofluorescence for diagnosing chlamydia infections]
Abstract:
The diagnostic value of indirect immunofluorescence in the detection of antichlamydial IgG antibodies in patients with chronic chlamydial salpingitis is insufficient. The condition may be diagnosed if antichlamydial IgG antibodies are detectable in the serum diluted at least 1:128. Examinations of paired sera are of no value in chronic chlamydial salpingitis.
Related Concept Videos
Immunofluorescence Microscopy
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...
The...
Immunocytochemistry and Immunohistochemistry
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
These...

