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Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Network Covalent Solids02:18

Network Covalent Solids

Network covalent solids contain a three-dimensional network of covalently bonded atoms as found in the crystal structures of nonmetals like diamond, graphite, silicon, and some covalent compounds, such as silicon dioxide (sand) and silicon carbide (carborundum, the abrasive on sandpaper). Many minerals have networks of covalent bonds.
To break or to melt a covalent network solid, covalent bonds must be broken. Because covalent bonds are relatively strong, covalent network solids are typically...

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Related Experiment Video

Updated: Jul 17, 2026

Pattern-based Search of Epigenomic Data Using GeNemo
06:38

Pattern-based Search of Epigenomic Data Using GeNemo

Published on: October 8, 2017

Improving e-book access via a library-developed full-text search tool.

Jill E Foust1, Phillip Bergen, Gretchen L Maxeiner

  • 1jef2@pitt.edu

Journal of the Medical Library Association : JMLA
|January 26, 2007
PubMed
Summary

A new federated search tool allows users to find information across over 2,500 electronic book (e-book) titles. This innovative system provides integrated results and categorized groupings for efficient information retrieval.

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Comparing Bibliometric Analysis Using PubMed, Scopus, and Web of Science Databases
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Comparing Bibliometric Analysis Using PubMed, Scopus, and Web of Science Databases

Published on: October 24, 2019

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Pattern-based Search of Epigenomic Data Using GeNemo
06:38

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Comparing Bibliometric Analysis Using PubMed, Scopus, and Web of Science Databases
05:02

Comparing Bibliometric Analysis Using PubMed, Scopus, and Web of Science Databases

Published on: October 24, 2019

Area of Science:

  • Library and Information Science
  • Digital Humanities
  • Health Sciences Informatics

Background:

  • The Health Sciences Library System (HSLS) supports the University of Pittsburgh's medical programs and health system.
  • Effective access to digital resources is crucial for academic and health institutions.

Purpose of the Study:

  • To report the development of a novel tool for searching licensed full-text electronic book (e-book) collections.
  • To enhance information retrieval within large digital library resources.

Main Methods:

  • Development of a federated search tool using the Vivísimo XML-based environment.
  • Integration of over 2,500 e-book titles from seven key collections.
  • Implementation of a single query interface with categorized, integrated results.

Main Results:

  • A heuristic evaluation confirmed the tool's usability.
  • Web server log analysis indicated acceptable user engagement.
  • The tool successfully enables full-text searching across diverse e-book collections.

Conclusions:

  • The developed federated search tool is effective for large-scale e-book collection searching.
  • This project provides a strong foundation for future library-based federated search products.
  • The tool enhances access to critical information within health sciences e-book resources.