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Related Experiment Videos

Dimensional changes in cells and tissues during specimen preparation for the electron microscope.

M V King1

  • 1Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.

Cell Biophysics
|February 1, 1991
PubMed
Summary

This review examines dimensional changes in tissue specimens during electron microscopy preparation. It offers methods for monitoring these changes and selecting optimal specimen preparation techniques for accurate morphometry.

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Area of Science:

  • Electron microscopy
  • Biological specimen preparation
  • Cell biology

Background:

  • Accurate morphometry and 3D reconstruction in electron microscopy require precise understanding of tissue specimen dimensional changes.
  • Various preparation techniques can induce significant alterations in specimen size and shape.
  • Quantitative data on these changes are crucial for reliable interpretation of ultrastructural studies.

Purpose of the Study:

  • To review studies on dimensional changes during tissue specimen preparation for electron microscopy.
  • To emphasize quantitative measurements relevant to morphometry and 3D reconstruction.
  • To provide recommendations for monitoring dimensional changes and selecting preparation methods.

Main Methods:

  • Review of literature on specimen preparation techniques including fixation, dehydration, plastic embedment, critical-point drying, and freeze-drying.

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  • Focus on studies reporting quantitative measurements of dimensional alterations.
  • Analysis of strategies for minimizing and monitoring these changes.

    • Main Results:

    • Dimensional changes occur at multiple stages of specimen preparation, including fixation and dehydration.
    • Techniques like critical-point drying and freeze-drying aim to minimize structural distortion but can introduce their own artifacts.
    • Quantitative data reveal significant variability in dimensional changes depending on the method and tissue type.

    Conclusions:

    • Consistent monitoring of dimensional changes is essential for accurate morphometric analysis.
    • A strategic approach to selecting specimen preparation methods based on tissue type and research goals is recommended.
    • Further research quantifying dimensional changes across a wider range of tissues and methods is warranted.