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Updated: Jul 17, 2026

Natural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis
Published on: May 31, 2019
Structural characterization of microcystins by LC/MS/MS under ion trap conditions
Tsuyoshi Mayumi1, Hajime Kato, Susumu Imanishi
1Graduate School of Environmental and Human Science & Faculty of Pharmacy, Meijo University, Tempaku, Nagoya 468-8503, Japan.
Abstract:
LC/MS/MS under ion trap conditions was used to analyze microcystins produced by cyanobacteria. Tandem mass spectrometry using MS2 was quite effective since ions arising from cleavage at a peptide bond provide useful sequence information. The fragmentation was confirmed by a shifting technique using structurally-related microcystins and the resulting fragmentation pattern was different from those determined by triple stage MS/MS and four sector MS/MS. Analysis of a mixture of microcystins in a bloom sample was successfully performed and two new microcystins were identified by LC/MS/MS under ion trap conditions. Thus, LC/MS/MS under ion trap conditions is effective for the structural characterization of microcystins.
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