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[Virus inactivation of plasma]
H Schmitt1, H Mohr, B Lambrecht
1Zentralinstitut Springe, DRK-Blutspendedienst Niedersachsen, BRD.
Beitrage Zur Infusionstherapie = Contributions to Infusion Therapy
|January 1, 1991
Summary
This study presents a novel virus inactivation method for plasma using phenothiazine dyes and visible light. The procedure effectively inactivates viruses like HIV while preserving plasma
Area of Science:
- Biochemistry
- Virology
- Hematology
Context:
- Plasma transfusions carry a risk of viral transmission.
- Existing virus inactivation methods can affect plasma component efficacy.
- Need for safe and effective viral clearance techniques in blood products.
Purpose:
- To describe a new method for virus inactivation in single-donor plasma.
- To evaluate the efficacy of phenothiazine dye and visible light treatment against viruses.
- To assess the impact of this inactivation process on plasma's biological activities and safety.
Summary:
- A procedure combining phenothiazine dyes (methylene blue, toluidine blue) with visible light inactivates viruses such as VSV, HSV, and HIV in plasma.
- This method results in minimal reduction of coagulation factors and inhibitors.
- No neo-antigens or IgE antibodies were detected, and autologous plasma showed no toxicity in canine studies.
Impact:
- Offers a potentially safer plasma transfusion option by reducing viral risk.
- Preserves critical plasma functions, maintaining therapeutic value.
- Demonstrates a well-tolerated virus inactivation technique with no adverse immunological reactions.