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Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
Published on: January 7, 2019
CD25-expressing B-lymphocytes in rheumatic diseases.
S Amu1, K Strömberg, M Bokarewa
1Department of Rheumatology and Inflammations Research, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden.
Scandinavian Journal of Immunology
|January 30, 2007
Summary
Activated B cells expressing CD25 are implicated in autoimmune diseases like rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). These B cells show a mature phenotype, suggesting a role in disease development.
Area of Science:
- Immunology
- Autoimmunity
- Cell Biology
Background:
- B cells are crucial in autoimmune diseases, producing autoantibodies and inflammatory cytokines.
- Rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) are autoimmune conditions with complex B cell involvement.
Purpose of the Study:
- To analyze B cells from RA and SLE patients for the expression of the IL-2 receptor (IL-2R) subunit CD25.
- To investigate the phenotype and activation state of CD25-expressing B cells in RA and SLE.
Main Methods:
- Flow cytometry was used to assess B cell surface marker expression.
- Comparison of CD25(+) B cells from RA and SLE patients with healthy controls.
Main Results:
- CD25(+) B cells in RA patients showed higher CD122 and CD132 expression, indicating a functional IL-2R.
- These RA B cells also had increased CD80 but decreased IgM and IgA expression.
- SLE B cells exhibited co-expression of CD25 with CD80, CD122, and CD132, alongside reduced IgD and IgM.
Conclusions:
- CD25(+) B cells in RA and SLE patients are highly activated and exhibit a mature phenotype.
- This activated B cell subset may play a significant role in the pathogenesis of RA and SLE.
- Findings highlight CD25(+) B cells as potential therapeutic targets in autoimmune diseases.
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