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Detection and Isolation of Apoptotic Bodies to High Purity
12:17

Detection and Isolation of Apoptotic Bodies to High Purity

Published on: August 12, 2018

Screening and detection of apoptosis

Sergio Huerta1, Emily J Goulet, Sara Huerta-Yepez

  • 1UT Southwestern Medical Center/VA North Texas Health Care System, Department of Gastrointestinal and Endocrine Surgery, Dallas, Texas, USA. Sergio.Huerta@UTSouthwestern.edu

The Journal of Surgical Research
|January 30, 2007
PubMed

Insights

This review examines methods for studying programmed cell death, also known as apoptosis. It details various assays, reagents, and molecules used to investigate this cell death pathway, highlighting pros and cons.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Programmed cell death, or apoptosis, has been recognized for over 30 years.
  • Apoptosis is a key target for understanding and treating diseases like cancer.
  • Techniques for studying apoptosis have significantly evolved since its initial description.

Purpose of the Study:

  • To review and summarize the methodologies used for studying apoptosis.
  • To provide an overview of various assays, reagents, and molecules employed in apoptosis research.
  • To critically evaluate the advantages and disadvantages of different apoptosis detection methods.

Main Methods:

  • A MEDLINE search of English-language literature was conducted.
  • The review focuses on established and emerging techniques for apoptosis analysis.
  • Information was synthesized to compare and contrast different study approaches.

Main Results:

  • A wide array of techniques exist for the study of apoptosis.
  • Each method possesses unique strengths and limitations.
  • Understanding these differences is crucial for selecting appropriate experimental approaches.

Conclusions:

  • The choice of method depends on the specific research question and experimental context.
  • Accurate assessment of apoptosis is vital for advancing cancer research and therapy.
  • Continued development of novel techniques will further enhance our understanding of programmed cell death.

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