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Related Experiment Videos

A quick-mixed aluminum hematoxylin stain.

E T Graham1

  • 1Department of Ornamental Horticulture, University of Tennessee, Knoxville 37901.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
|January 1, 1991
PubMed
Summary

A new aluminum-hematein stain was developed for plant tissues. This ready-to-use stain, prepared in small volumes, performs well in paraffin sections, offering a convenient staining solution.

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Area of Science:

  • Plant biology
  • Histology
  • Staining techniques

Background:

  • Hematoxylin and aluminum stains are common in histology.
  • Propylene glycol offers a less toxic alternative solvent.

Purpose of the Study:

  • To develop a novel, ready-to-use aluminum-hematein stain.
  • To evaluate the stain's performance in plant tissue sections.

Main Methods:

  • Two stock solutions were prepared: A (aluminum sulfate, sodium iodate, acetic acid in aqueous propylene glycol) and B (hematoxylin in propylene glycol).
  • Solutions A and B were combined in specified proportions to create the final stain.
  • The stain was applied to paraffin sections of plant tissues.

Main Results:

  • The combined solutions yielded aluminum-hematein dissolved in propylene glycol.
  • The ready-to-use stain performed well in paraffin sections of plant tissues.
  • The stain is prepared in small volumes as needed.

Conclusions:

  • A stable and effective aluminum-hematein stain was successfully developed.
  • The stain is suitable for routine use in plant histology.
  • The use of propylene glycol ensures a nontoxic staining solution.

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