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Related Experiment Videos

Androgen responsive intronic non-coding RNAs.

Rodrigo Louro1, Helder I Nakaya, Paulo P Amaral

  • 1Departamento de Bioquimica, Instituto de Quimica, Universidade de São Paulo, 05508-900 São Paulo, Brazil. rolouro@iq.usp.br

BMC Biology
|February 1, 2007
PubMed
Summary
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Androgen hormones regulate numerous intronic non-coding RNAs in prostate cancer cells. These findings suggest shared regulatory mechanisms and functional roles for intronic RNAs in gene expression.

Area of Science:

  • Molecular Biology
  • Genomics
  • Cancer Research

Background:

  • Recent reports indicate transcription of numerous non-coding RNAs from human gene introns.
  • Mechanisms governing intronic non-coding RNA biosynthesis and function remain largely unknown.
  • Investigating shared transcriptional regulation between protein-coding mRNAs and intronic RNAs is crucial.

Purpose of the Study:

  • To evaluate a common transcription regulation mechanism for protein-coding mRNAs and intronic RNAs.
  • To measure the effect of androgen on the transcriptional profile in a prostate cancer cell line.

Main Methods:

  • Utilized a custom-built cDNA microarray enriched in intronic transcribed sequences.
  • Performed orientation-specific reverse transcription-PCR to identify transcript directionality.

Related Experiment Videos

  • Employed chromatin immunoprecipitation to confirm androgen receptor binding.

    • Main Results:

    • Identified 39 intronic non-coding RNAs significantly regulated by androgen exposure.
    • Found 10 of 13 analyzed transcripts were in the antisense direction, long (0.5-5 kb), and unspliced.
    • Correlated androgen-regulated intronic transcript levels with corresponding protein-coding gene levels or alternative exon usage.

    Conclusions:

    • A fraction of naturally transcribed intronic non-coding RNAs are regulated by physiological signals like hormones.
    • These findings support the notion that intronic RNAs play functional roles in human gene expression.
    • Suggests shared regulatory pathways for protein-coding and intronic RNAs.