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Updated: Jul 17, 2026
Nitropeptide Profiling and Identification Illustrated by Angiotensin II
Published on: June 16, 2019
[The N-terminal pro-brain natriuretic peptide (NT-proBNP) assay with the Stratus CS analyzer]
C Chenevier-Gobeaux1, D Guillot, J Ursulet
1Laboratoire de Biochimie A, Hôpital Cochin, Assistance Publique - Hôpitaux de Paris (AP-HP), Paris. camille.gobeaux@cch.ap-hop-paris.fr
Background:
NT-proBNP is an efficient biomarker for the evaluation, management and prognosis of patients with heart failure.
Methods:
We evaluated the analytical performance of the NT-proBNP immunoenzymatic assay with the Stratus CS semi-automated analyzer in two hospital laboratories. The characteristics assessed included imprecision, functional sensitivity, linearity/recovery, interferences study, high-dose hook effect and a comparison of Acute Care(TM) pPBNP (on Stratus)CS) versus PBNP (on Dimension HM) results on patient heparinized plasma samples.
Results:
Total imprecision reached < 5% coefficient of variation at NT-proBNP concentrations of 186-19,649 ng/L; recovery values for diluted samples were between 89.0 and 110.0 %; functional sensitivity reached 21 ng/L; there was no high-dose hook effect at concentrations up to 400,000 ng/L; hemaoglobin affected negatively but <10% the NT-proBNP assay, while bilirubin and triglycerides did not affected it more than 5%; Stratus CS results were strongly correlated with Dimension HM results (R(2)=1,00).
Conclusion:
The Stratus CS Acute Care pPBNP assay demonstrated excellent analytical performance which agreed with the Dimension HM PBNP assay. This analyzer is therefore suitable for use by low NT-proBNP test volume laboratories, and also by Emergency departments and Intensive care units.
