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Current role of LC-MS(/MS) in doping control.

Mario Thevis¹, Wilhelm Schänzer

¹Center for Preventive Doping Research - Institute of Biochemistry, German Sport University Cologne, Carl-Diem-Weg 6, 50933 Cologne, Germany. m.thevis@biochem.dshs-koeln.de

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Summary

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has transformed sports drug testing. This advanced technique allows for the detection of previously undetectable substances, improving the accuracy and efficiency of anti-doping analysis.

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Area of Science:

Analytical Chemistry
Sports Science
Biochemistry

Background:

Traditional doping control methods faced limitations in detecting certain substances.
Advancements in analytical techniques were needed to enhance anti-doping capabilities.
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) emerged as a powerful tool.

Purpose of the Study:

To provide a critical overview of LC-MS/MS contributions to sports drug testing.
To highlight recent developments in detecting low and high molecular weight drugs.
To assess the impact of LC-MS/MS on doping control analysis.

Main Methods:

Review of recent scientific literature on LC-MS/MS in doping control.
Analysis of new methods for drug determination in sports samples.
Comparison of LC-MS/MS with alternative chromatographic and immunochemical techniques.

Main Results:

LC-MS/MS has revolutionized doping control assays over the past decade.
New methods enable the determination of previously undetectable drugs.
Faster, more comprehensive, and robust assays have replaced complex procedures.

Conclusions:

LC-MS/MS significantly enhances the detection capabilities in sports drug testing.
The technique has streamlined and improved the overall efficiency of anti-doping analysis.
Ongoing developments continue to expand the scope of detectable substances in sports.