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Assessing the Quantitative Performance of Atmospheric Solids Analysis Probe-Mass Spectrometry.

Alisha Henderson1, Stephanie Rankin-Turner2, James C Reynolds3

  • 1School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, UK.

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PubMed
Summary
This summary is machine-generated.

Atmospheric solids analysis probe-mass spectrometry (ASAP-MS) can provide quantitative data with optimized methods. Using a specific pipette and internal standard improves reliability, though sensitivity limitations exist below 1000 ng/mL.

Keywords:
ASAP‐MSaccuracyambient ionisation mass spectrometryprecisionquantitation

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Ambient Ionization Techniques

Background:

  • Atmospheric solids analysis probe-mass spectrometry (ASAP-MS) is an ambient ionization technique for rapid sample analysis without chromatography.
  • ASAP-MS offers potential for increased sample throughput and in situ testing.
  • Previous studies have shown conflicting results regarding the quantitative reliability of ASAP-MS, with a lack of in-depth data.

Purpose of the Study:

  • To comprehensively assess the quantitative performance of ASAP-MS.
  • To identify optimal conditions for reliable quantitative analysis using ASAP-MS.
  • To provide practical recommendations for improving quantitative ASAP-MS applications.

Main Methods:

  • Experiments were conducted using caffeine as a model analyte to evaluate quantitative performance.
  • Key variables investigated included sample deposition methods (direct probe vs. pipetting) and deposition volumes.
  • The impact of various internal standard approaches on analytical precision, accuracy, linearity, and sensitivity was assessed.

Main Results:

  • Optimal quantitative reliability was achieved using a positive displacement pipette and an isotopically labeled internal standard.
  • Measurable responses were observed across the 50-5000 ng/mL concentration range.
  • Sensitivity limitations were noted, with reduced quantitative performance below 1000 ng/mL.

Conclusions:

  • This study provides the first comprehensive investigation into the quantitative capabilities of ASAP-MS.
  • The findings highlight the strengths and limitations of quantitative analysis using ASAP-MS.
  • Recommendations are offered to optimize quantitative approaches for ASAP-MS, enhancing its utility in various applications.