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Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
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Meat Speciation via Deployable Atmospheric Solid Analysis Probe Mass Spectrometry (ASAP-MS) Using Prototype

Vanshni Vekereya1, Rebecca Nash1, Valerio Converso1

  • 1National Measurement Laboratory, LGC, Guildford, UK.

Rapid Communications in Mass Spectrometry : RCM
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PubMed
Summary
This summary is machine-generated.

A new method using Atmospheric Solid Analysis Probe Mass Spectrometry (ASAP-MS) can rapidly and accurately detect meat adulteration. This technique offers a fast, reliable tool for verifying meat authenticity in various settings.

Keywords:
ASAP‐MSPCA‐LDAfood fraudlipid profilingmeat speciationportable mass spectrometry

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Area of Science:

  • Food Science
  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Food fraud, specifically meat adulteration, erodes consumer trust and poses public health risks.
  • Current detection methods for meat authenticity are often time-consuming and require extensive sample preparation.
  • There is a critical need for swift, dependable, and accessible analytical techniques for meat verification.

Purpose of the Study:

  • To develop and validate a rapid analytical method for identifying meat species and detecting adulteration.
  • To assess the feasibility of using Atmospheric Solid Analysis Probe Mass Spectrometry (ASAP-MS) for direct meat sample analysis.
  • To implement chemometric modelling for accurate classification of meat types and adulteration levels.

Main Methods:

  • Atmospheric Solid Analysis Probe Mass Spectrometry (ASAP-MS) was employed for direct analysis of meat samples with minimal pre-treatment.
  • Spectral data from seven distinct meat species were analyzed using chemometric modeling to establish a robust classification system.
  • The developed model was rigorously validated with laboratory-prepared mixed meat samples and subsequently tested on commercial food products.

Main Results:

  • The ASAP-MS chemometric model accurately differentiated between seven meat species and detected adulteration down to 5% in mixed samples.
  • Sample analysis was completed in under 5 minutes, showcasing significant speed improvement over traditional methods.
  • Application to commercial processed foods and ready meals resulted in 100% agreement with stated labels, confirming high accuracy and reliability.

Conclusions:

  • Atmospheric Solid Analysis Probe Mass Spectrometry (ASAP-MS) coupled with chemometric modeling offers a fast, accurate, and minimally invasive solution for meat speciation.
  • The method's speed and potential for portability make it ideal for real-time, on-site food authenticity testing.
  • This approach provides a valuable tool for enhancing food authenticity monitoring, safeguarding consumers, and ensuring regulatory compliance.