Related Experiment Video
Updated: Jul 17, 2026
Experimental Model to Evaluate Resolution of Pneumonia
Published on: February 17, 2023
Analysis of pulmonary mechanics in an experimental model of sepsis
Rodrigo Storck Carvalho1, André Gustavo Magalhães de Pinho, Ana Paula Alves de Andrade
1Universidade de Brasília, Brasília, DF, Brasil. rodrigo.storck@terra.com.br
Objective:
To determine whether pulmonary mechanics are altered in mice with sepsis.
Methods:
A total of 40 Balb/c mice were divided into two groups: survival (n = 21) and pulmonary mechanics (n = 19). The survival group was divided into three subgroups: control (n = 7), sublethal (n = 7) and lethal (n = 7). The pulmonary mechanics group was also divided into three subgroups: control (n = 5), sublethal (n = 7) and lethal (n = 7). Sepsis was induced through cecal ligation and puncture, the latter varying in degree (sublethal or lethal). At eight hours after the intervention, pulmonary mechanics were measured through end-inflation occlusion. In the pulmonary mechanics group, the following variables were studied: total pressure, resistance, viscoelasticity, dynamic compliance and static compliance. The data obtained were analyzed using one-way ANOVA.
Results:
The data for the survival group indicate the efficacy of the model employed. There were no statistically significant differences among the pulmonary mechanics subgroups in terms of dynamic compliance, static compliance, total pressure, resistance or viscoelasticity.
Conclusion:
At eight hours after cecal ligation and puncture, there were no changes in the lung parenchyma, nor were any alterations observed in the viscous and viscoelastic components of the lung.
