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Measuring In Vitro ATPase Activity for Enzymatic Characterization
Published on: August 23, 2016
Supplementation effect of ectoine on thermostability of phytase
Linghua Zhang1, Yue Wang, Chunyu Zhang
1College of Environmental Science and Engineering, Dalian Maritime University, Lingshui Road, Dalian 116026, P.R. China.
Abstract:
In this study, we elucidated the supplementation effect of compatible solutes on the thermostability of phytase, designated as PHYA II, which was encoded by the phytase gene phyA I (GeneBank AY013315) from Aspergillus ficuum As3.324 and expressed in Pichia pastoris GS115. When PHYA II in acetate buffer was heated at 90 degrees C for 15 min, more than 80% of the residual activity was retained by adding the cyclic amino acid ectoine, a representative compatible solute. Furthermore, the presence of ectoine led to an increase in the relative hydrolytic rate of sodium phytate by 15.7% with heating at 80 degrees C for 15 min. Among the compatible solutes examined, ectoine was confirmed to be the most efficient thermoprotectant for PHYA II.
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