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Expression of Aspergillus oryzae phytase gene in Aspergillus oryzae RIB40 niaD(-)
Hiroyuki Uchida1, Shinya Arakida, Tatsuji Sakamoto
1Department of Applied Chemistry and Biotechnology, Faculty of Engineering, University of Fukui, 9-1 Bunkyo 3-chome, Fukui-shi 910-8507, Japan. uchida@acbio.fukui-u.ac.jp
Abstract:
Aspergillus oryzae RIB40 niaD(-) was transformed using a plasmid constructed with the A. oryzae phytase gene and pNAN8142 vector. The culture broth of the transformant, which was grown in a medium containing starch as a carbon source and polyvinylpyrrolidone showed phytase activity of a maximum of 2.0 units ml(-1) at 37 degrees C, pH 5.5.
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