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MEMS-based flow cytometry: microfluidics-based cell identification system by fluorescent imaging.

W K Wu1, C K Liang, J Z Huang

  • 1Dept. of Electr. Eng., Southern Taiwan Univ. of Tech., Taipei, Taiwan.

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
|February 3, 2007
PubMed
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This study presents a novel, low-cost microfluidics biochip system using MEMS technology for precise cell handling. The system successfully separates yeast and PC-3 cells, demonstrating its potential for biological analysis.

Area of Science:

  • Biotechnology
  • Microfluidics
  • MEMS technology

Background:

  • Cell handling and analysis are crucial in biological research.
  • Existing methods can be costly and complex.
  • There is a need for integrated, low-cost biochip systems.

Purpose of the Study:

  • To develop a novel, low-cost microfluidics-based biochip system for flow-type cell handling.
  • To enable precise cell manipulation and detection using MEMS technology.
  • To demonstrate the system's capability in cell separation and identification.

Main Methods:

  • Utilized Micro-Electro-Mechanical Systems (MEMS) technology for biochip fabrication.
  • Implemented a microfluidic driving system powered by a vacuum pump.
  • Employed sheath flow prefocus for ordered, single-file cell movement.

Related Experiment Videos

  • Integrated a fluorescent inspection region for cell image identification and counting.

    • Main Results:

    • Successfully manufactured a glass-based biochip and set up the complete system.
    • Demonstrated effective cell separation of yeast and PC-3 cells.
    • Presented separated cell images, validating the system's performance.

    Conclusions:

    • The developed microfluidics biochip system offers a low-cost solution for flow-type cell handling.
    • The system enables precise cell manipulation, separation, and analysis.
    • This technology holds promise for advancing cell-based assays and diagnostics.