Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Optimization of multi-frequency techniques used for cell membrane capacitance estimation.

S F Lempka1, D W Barnett

  • 1Dept. of Biomed. Eng., Saint Louis Univ., St. Louis, MO, USA.

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
|February 3, 2007
PubMed
Summary

This study introduces an optimized multi-frequency stimulus waveform to precisely measure cell membrane capacitance changes, crucial for monitoring cell secretion processes like exocytosis and endocytosis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional Somatotopy of Lumbar Dorsal Rootlets and its Role in Selective Recruitment via Lateral Spinal Cord Stimulation.

bioRxiv : the preprint server for biology·2026
Same author

A finite element model of electrode placement during stimulus evoked electromyographic monitoring of iliosacral screw insertion.

The open orthopaedics journal·2009
Same author

Perinatal herpes simplex infection presenting with pneumonia and pleural effusions.

The Pediatric infectious disease journal·2001
Same author

An optimized approach to membrane capacitance estimation using dual-frequency excitation.

Biophysical journal·1997
Same author

Single-cell measurements of quantal secretion induced by alpha-latrotoxin from rat adrenal chromaffin cells: dependence on extracellular Ca2+.

Pflugers Archiv : European journal of physiology·1996
Same author

Voltage-dependent Na+ and Ca2+ currents in human pancreatic islet beta-cells: evidence for roles in the generation of action potentials and insulin secretion.

Pflugers Archiv : European journal of physiology·1995

Area of Science:

  • Cell Biology
  • Biophysics
  • Electrophysiology

Background:

  • Cell membrane capacitance measurements are vital for studying stimulus-secretion coupling, reflecting cell membrane surface area changes during exocytosis and endocytosis.
  • Accurate monitoring of vesicle trafficking requires high-resolution detection of minute capacitance fluctuations (10⁻¹⁵ F).
  • Existing capacitance measurement techniques often rely on limiting assumptions or exhibit a low signal-to-noise ratio (SNR).

Purpose of the Study:

  • To develop an improved method for accurate cell capacitance estimation.
  • To enhance the signal-to-noise ratio (SNR) in capacitance measurements.
  • To optimize stimulus waveforms for precise monitoring of exocytosis and endocytosis.

Main Methods:

  • Design and implementation of an optimized multi-frequency sinusoidal stimulus waveform.

Related Experiment Videos

  • Joint optimization of phase and voltage for individual frequency components.
  • Minimization of the theoretical variance bound for capacitance estimates under a constrained stimulus magnitude.

    • Main Results:

    • The proposed optimized waveform allows for more accurate capacitance estimations compared to current methods.
    • The technique effectively minimizes the variance in capacitance measurements.
    • The method maintains a controlled overall stimulus magnitude.

    Conclusions:

    • The developed multi-frequency waveform offers a superior approach for high-resolution cell capacitance measurements.
    • This advancement improves the reliability of monitoring dynamic cell membrane processes like exocytosis and endocytosis.
    • The optimized method addresses limitations of existing techniques, enhancing accuracy and SNR.