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Purification of neuropathy target esterase from avian brain after prelabelling with [3H]diisopropyl
M E Rüffer-Turner1, D J Read, M K Johnson
1MRC Toxicology Unit, Medical Research Council Laboratories, Surrey, England, U.K.
Journal of Neurochemistry
|January 1, 1992
Abstract:
Neuropathy target esterase from hen brains was radiolabelled at the active site with [3H]diisopropyl phosphorofluoridate. The labelled protein was purified by differential centrifugation and Nonidet P40 solubilization, detergent phase partitioning, anion exchange, and preparative sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The volatilizable counts assay and analytical SDS-PAGE were used to monitor the protein. The 150-kDa subunit polypeptide appears as a single band on analytical SDS-PAGE.