Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Apoptosis-induced decrease of intrathyroidal CD4(+)CD25(+) regulatory T cells in autoimmune thyroid diseases.

Aiko Nakano1, Mikio Watanabe, Takao Iida

  • 1Division of Biomedical Informatics, Course of Health Science, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.

Thyroid : Official Journal of the American Thyroid Association
|February 6, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association Between Workplace Social Support and Use of Health-Promoting Wearable Devices: A Prospective Cohort Study of Japanese Employees.

Journal of UOEH·2023
Same author

Comparisons between tumor burden and other prognostic factors that influence survival of patients with non-small cell lung cancer treated with immune checkpoint inhibitors.

Thoracic cancer·2019
Same author

The association between thyroid hormone balance and thyroid volume in patients with Hashimoto thyroiditis.

Endocrine journal·2019
Same author

Exploratory analysis of prognostic factors for lenvatinib in radioiodine-refractory differentiated thyroid cancer.

Head & neck·2019
Same author

Ultrasound image analysis using deep learning algorithm for the diagnosis of thyroid nodules.

Medicine·2019
Same author

A Novel Diagnostic Method for Thyroid Follicular Tumors Based on Immunofluorescence Analysis of p53-Binding Protein 1 Expression: Detection of Genomic Instability.

Thyroid : official journal of the American Thyroid Association·2019

In autoimmune thyroid disease (AITD), fewer regulatory T (Treg) cells are found in the thyroid due to increased apoptosis. This reduction may impair the regulation of autoreactive T cells, contributing to the disease.

Area of Science:

  • Immunology
  • Endocrinology
  • Autoimmune Diseases

Background:

  • Previous studies indicated lower CD4(+) T cell proportions and higher Fas expression in the thyroid of autoimmune thyroid disease (AITD) patients.
  • Fas expression on intrathyroidal CD4(+) T cells was elevated compared to peripheral blood.

Purpose of the Study:

  • To investigate if intrathyroidal CD4(+)CD25(+) regulatory T (Treg) cells are reduced by Fas-mediated apoptosis in AITD patients.
  • To clarify the role of apoptosis in Treg cell depletion within the thyroid gland in AITD.

Main Methods:

  • Three-color flow cytometry was used to analyze intrathyroidal and peripheral lymphocytes.
  • Samples were obtained from 20 AITD patients (Graves' disease and Hashimoto's disease) and 10 healthy volunteers.

Related Experiment Videos

Main Results:

  • The proportion of CD4(+)CD25(+) cells, including specific Treg subsets (CD4(+)CD25(+)CD69() and Foxp3(+)CD4(+)CD25(+)), was lower in the thyroid of AITD patients compared to their peripheral blood and healthy controls.
  • A higher proportion of apoptotic cells was observed among intrathyroidal CD4(+) cells and intrathyroidal CD4(+)CD25(+) cells compared to peripheral CD4(+) cells and intrathyroidal CD4(+)CD25() cells, respectively.

Conclusions:

  • Intrathyroidal Treg cells are decreased in AITD patients due to apoptosis.
  • This reduction in Treg cells may lead to incomplete regulation of autoreactive T cells, contributing to AITD pathogenesis.