Therapeutic protein production in vivo after electroporation-assisted intramuscular gene delivery

Gene delivery to skeletal muscles assisted by electroporation(EP) is a promising strategy for in vivo production of the therapeutic proteins. But the commonly used procedure required the application of electric pulses with voltages at above 200V/cm and durations at least 40ms, which would result in several damages in the muscle and limited surviving cells expressing transgene. We reported here an optimization study of the various electric pulse parameters to reduce toxicity while maintain transgene expression. In addition, we also found that the secreted transgene product level detected in serum samples may not correlate with the total gene expression level in muscles. Based on our data, we'd propose some less damaging electroporation parameters that may be useful for intramuscular gene delivery and therapeutic protein production.