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Ice formation of vitrification solutions for cryopreservation of tissues

B L Liu1, J J McGrath

  • 1Institute of Cryomedicine, Shanghai University of Science and Technology, Shanghai, 200093, China, Department of Aerospace and Mechanical Engineering, University of Arizona, Tucson, AZ 85721, USA.

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
|February 7, 2007
PubMed

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...