Fabricating nanofluidic channels and Applying it for single bio-molecule study

In the emerging field of nanobiotechnology, further downsizing the fluidic channels to the nanometer scale is attractive for both fundamental studies and technical applications. The insulation Silicon nitride membrane nanofluidic channel array which have width∼75nm and depth ∼ 100nm and length 50μm were created by focused-ion- beam instrument, theλ--DNA molecules were put inside them and the dynamic characteristics were initial studied, a fluorescence microscopy was used to observe the images. We observed λ--DNA moved inside the nanotrenches which dealt with activating reagent Brij aqueous solution only by capillary force,this will help us to understand more DNA dynamics characteristics and more information about single biomolecule transporting through a nanopore,.