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Updated: Jul 17, 2026

One-step Extraction and Zymographic Analysis of Bacterial Gelatinases
Published on: August 1, 2025
Study of gelatin-agar intermolecular aggregates in the supernatant of its coacervate
S Santinath Singh1, H B Bohidar, S Bandyopadhyay
1Polymer and Biophysics Laboratory, School of Physical Sciences, Jawaharlal Nehru University, New Delhi 110016, India.
Abstract:
Intermolecular interaction leading to formation of aggregates between gelatin, a polyampholyte, and agar, a polysaccharide was studied in the supernatant of the complex coacervate formed by these biopolymers. Electrophoresis, laser light scattering and viscometry data were used to determine the interaction and the physical structure of these intermolecular soluble complexes by modeling these to be prolate ellipsoids of revolution (rod-like structures with well defined axial ratio and Perrin's factor). Solution ionic strength was found to reduce the axial ratio of these complexes implying the presence of screened polarization-induced electrostatic interaction between the two biopolymers.
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