Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Single biomolecule imaging with frequency and force modulation in tapping-mode atomic force microscopy.

Santiago D Solares

    The Journal of Physical Chemistry. B
    |February 13, 2007
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Probe assisted localized doping of aluminum into silicon substrates.

    Journal of applied physics·2024
    Same author

    Cell Membrane Oscillations under Radiofrequency Electromagnetic Modulation.

    Langmuir : the ACS journal of surfaces and colloids·2023
    Same author

    Frequency-dependent nanomechanical profiling for medical diagnosis.

    Beilstein journal of nanotechnology·2022
    Same author

    Optimizing the accuracy of viscoelastic characterization with AFM force-distance experiments in the time and frequency domains.

    Soft matter·2022
    Same author

    Viscoelastic parameterization of human skin cells characterize material behavior at multiple timescales.

    Communications biology·2022
    Same author

    Corrosion Resistance of Sulfur-Selenium Alloy Coatings.

    Advanced materials (Deerfield Beach, Fla.)·2021

    A novel atomic force microscopy (AFM) technique, frequency and force modulation AFM (FFM-AFM), allows for non-damaging characterization of soft samples like single bacteriorhodopsin molecules. This method measures molecular cross sections and may enable quantitative nanoelasticity measurements.

    Area of Science:

    • Nanotechnology
    • Biophysics
    • Materials Science

    Background:

    • Atomic Force Microscopy (AFM) is crucial for nanoscale imaging.
    • Conventional AFM modes can damage soft biological samples.
    • Bistability and high tip-sample forces limit current AFM applications.

    Purpose of the Study:

    • To introduce and theoretically model a new AFM mode: frequency and force modulation AFM (FFM-AFM).
    • To demonstrate FFM-AFM's capability for non-damaging characterization of soft samples, specifically a single bacteriorhodopsin molecule.
    • To explore FFM-AFM's potential for quantitative nanoelasticity measurements.

    Main Methods:

    • Theoretical modeling of FFM-AFM.
    • Application of FFM-AFM principles to a single bacteriorhodopsin molecule on a substrate.

    Related Experiment Videos

  • Utilizing excitation force frequency and amplitude modulation to overcome limitations of conventional AFM.

    • Main Results:

    • FFM-AFM can measure the cross section of a single bacteriorhodopsin molecule without causing damage.
    • The proposed method effectively eliminates bistability issues common in AFM.
    • Reduced tip-sample forces are achieved through modulated excitation.

    Conclusions:

    • FFM-AFM presents a promising advancement for the characterization of soft biological and nanoscale materials.
    • This technique offers a non-destructive alternative to conventional tapping-mode AFM.
    • Further research may establish FFM-AFM for precise quantitative nanoelasticity measurements.