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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jul 17, 2026

Novel Photoacoustic Microscopy and Optical Coherence Tomography Dual-modality Chorioretinal Imaging in Living Rabbit Eyes

Novel Photoacoustic Microscopy and Optical Coherence Tomography Dual-modality Chorioretinal Imaging in Living Rabbit Eyes

Published on: February 8, 2018

Development of a microarray chip for gene expression in rabbit ocular research.

Michael P Popp¹, Li Liu, Adrian Timmers

¹University of Florida's Interdisciplinary Center for Biotechnology Research, University of Florida, Gainsville, FL 32610-0294, USA. mpopp@obgyn.ufl.edu <mpopp@obgyn.ufl.edu>

Molecular Vision

|February 13, 2007

Summary

A new rabbit ocular microarray was developed for gene expression research. This tool validated wound healing responses, offering insights into molecular events in the eye.

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Area of Science:

Ophthalmology
Molecular Biology
Genomics

Background:

Ocular gene expression research requires specialized tools.
Rabbit models are crucial for studying eye diseases and treatments.

Purpose of the Study:

To develop a novel microarray for comprehensive ocular gene expression analysis in rabbits.
To create a valuable tool for investigating molecular mechanisms in the eye.

Main Methods:

cDNA libraries were constructed from rabbit ocular tissues.
Sequencing, assembly, and homology searches were performed to identify unique gene sequences.
Oligonucleotide probes were synthesized and arrayed for gene expression profiling.

Main Results:

A custom rabbit ocular microarray was successfully developed with 3,154 probes.
Gene expression analysis revealed significant alterations in 315 genes post-glaucoma filtration surgery.
Affected genes were associated with inflammatory response, defense mechanisms, and extracellular matrix synthesis.

Conclusions:

The developed rabbit microarray provides valid data on gene expression patterns, consistent with wound healing studies.
This represents the first available microarray for rabbit ocular research.
The microarray is a valuable tool for studying molecular events in the rabbit eye.