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Anti-Nuclear Antibody Screening Using HEp-2 Cells
Published on: June 23, 2014
[Serological screening of autoantigens associated with vasculitis in systemic lupus erythematosus]
Wei Qin1, Sheng-gang Feng, Hong-xin Deng
1Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To screen autoantigens associated with vasculitis in systemic lupus erythematosus (SLE) by serological analysis of recombinant cDNA expression library (SEREX).
Methods:
A cDNA expression library derived from human microvascular endothelial cells (HMVEC) was screened with pooled sera from patients with SLE. Pooled sera were preadsorbed to reduce the background sera activity. The obtained preadsorbed pooled sera of patients were used for immunoscreening. Immunoscreening procedure was done according to the instruction manual provided by the manufacturer (Stratagene).
Results:
Eleven distinct positive clones were identified. Bioinformatic analysis revealed these 11 positive clones encoding three different proteins: SSA, SSB and YB-1. As for YB-1, there are hitherto no reports unveiling its association with vasculitis in SLE.
Conclusion:
The association of YB-1 with vasculitis in SLE was found for the first time in this study. SEREX has offered a new strategy to explore the autoantigens related to SLE. By SEREX, we may find out the autoantigen which is helpful to the diagnosis and treatment of SLE, and to the prognostication concerned.
