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Related Concept Videos

Karyotyping01:17

Karyotyping

Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...
Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.

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Updated: Jul 17, 2026

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants
09:08

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

Published on: December 16, 2015

Chromosome-based genomics in the cereals.

Jaroslav Dolezel1, Marie Kubaláková, Etienne Paux

  • 1Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovská 6, CZ-77200, Olomouc, Czech Republic. dolezel@ueb.cas.cz

Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology
|February 14, 2007
PubMed
Summary

Flow cytometry enables the sorting of individual chromosomes from large cereal genomes. This chromosome-based approach overcomes challenges in sequencing complex plant genomes, aiding gene cloning and analysis.

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Area of Science:

  • Plant genomics
  • Cytogenetics
  • Bioinformatics

Background:

  • Cereal crops like wheat and maize possess large, complex genomes.
  • Large genomes present challenges for DNA sequencing and assembly due to repetitive DNA and polyploidy.
  • Traditional genetic mapping benefited from large chromosomes, but modern genomics requires genome dissection.

Purpose of the Study:

  • To review methods for dissecting complex cereal genomes using flow cytometric sorting of chromosomes.
  • To highlight the advantages of a chromosome-based approach for cereal genome analysis and gene cloning.
  • To demonstrate the utility of flow-sorted chromosomes in overcoming genomic complexities.

Main Methods:

  • Preparation of intact chromosome suspensions from major cereal species.
  • Analysis and sorting of chromosomes using flow cytometry.
  • Utilizing cytogenetic stocks (deletion and alien addition lines) to resolve specific chromosomes and arms in polyploid wheats and oats.

Main Results:

  • Successful development of methods for preparing and sorting cereal chromosomes.
  • Demonstration that flow cytometry can effectively discriminate and isolate individual chromosomes and chromosome arms.
  • Validation of a chromosome-based approach for analyzing complex cereal genomes.

Conclusions:

  • Flow cytometric chromosome sorting offers an effective solution for analyzing large and complex cereal genomes.
  • This technique facilitates genome sequencing, gene cloning, and detailed genetic analysis in crops like wheat and maize.
  • A chromosome-centric strategy is crucial for advancing cereal genomics and crop improvement.