Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Meiosis II01:57

Meiosis II

Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each containing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The CathPilot: Usability and Feasibility Evaluation in Simulated-Use Endovascular Interventions.

Annals of biomedical engineering·2026
Same author

The ABRF 2025 Annual Meeting: A Whirlwind of Core Facility Insights in the Electrifying Landscape of Las Vegas.

Journal of biomolecular techniques : JBT·2026
Same author

The Alternative Imaging Modalities in Ischemic Heart Failure (AIMI-HF) Trial-IMAGE HF Project 1A.

CJC open·2025
Same author

Modified Unipolar Return Pulsed Field Ablation in Ventricular Myocardium.

Circulation. Arrhythmia and electrophysiology·2025
Same author

Feasibility study: Characterizing acute lesion dimensions in patients with and without devices using noncontrast (native T<sub>1</sub>-weighted) magnetic resonance imaging after ventricular tachycardia/premature ventricular complex radiofrequency ablation.

Heart rhythm·2025
Same author

Biophysics and electrophysiology of pulsed field ablation in normal and infarcted porcine cardiac ventricular tissue.

Scientific reports·2024

Related Experiment Video

Updated: Jul 17, 2026

Freezing and Thawing Human Embryonic Stem Cells
08:49

Freezing and Thawing Human Embryonic Stem Cells

Published on: December 24, 2009

Lysed cell removal promotes frozen-thawed embryo development.

Thomas A Elliott1, Luiz Fernando A Colturato, Tyl H Taylor

  • 1Reproductive Biology Associates, Atlanta, Georgia 30342, USA. Thomas@ivf.net

Fertility and Sterility
|February 14, 2007
PubMed
Summary

Removing lysed cells from thawed mouse embryos significantly improves their developmental potential and morphology. This study validates mechanical lysis as a method to investigate frozen-thawed lysed cell removal in assisted reproduction.

More Related Videos

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
06:00

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

Published on: November 18, 2011

Freezing Human ES Cells
08:00

Freezing Human ES Cells

Published on: October 12, 2006

Related Experiment Videos

Last Updated: Jul 17, 2026

Freezing and Thawing Human Embryonic Stem Cells
08:49

Freezing and Thawing Human Embryonic Stem Cells

Published on: December 24, 2009

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
06:00

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

Published on: November 18, 2011

Freezing Human ES Cells
08:00

Freezing Human ES Cells

Published on: October 12, 2006

Area of Science:

  • Reproductive Biology
  • Embryology
  • Assisted Reproductive Technology

Background:

  • Cryopreservation of embryos is crucial in assisted reproductive technology.
  • The presence of lysed cells after thawing can negatively impact embryo development.
  • Understanding the effects of lysed cell removal is essential for improving IVF success rates.

Purpose of the Study:

  • To develop a mouse model to investigate the impact of lysed cell removal on thawed embryos.
  • To determine the causes of improved success rates when lysed cells are removed.

Main Methods:

  • Experimental study involving mouse embryos in a clinical IVF laboratory setting.
  • Assisted hatching, artificial cell lysis, and subsequent removal of lysed cells were performed.
  • Embryonic growth rate and morphology were assessed as primary outcome measures.

Main Results:

  • Embryonic development was significantly delayed in embryos with lysed cells only (Group 2) compared to controls and those with lysed cell removal (Group 1).
  • Embryo morphology on day 4 was significantly improved in Group 1 and the control group versus Group 2.
  • No significant difference in initial blastomere count or number of lysed cells between Group 1 and Group 2.

Conclusions:

  • Artificially lysed cells in thawed mouse embryos impair developmental quality and growth.
  • Removal of lysed cells restores the developmental potential and improves morphology, comparable to control embryos.
  • Mechanical lysis is a suitable method for further research into frozen-thawed lysed cell removal in human embryos.