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Published on: February 1, 2018
Amperometric ethanol biosensor based on poly(vinyl alcohol)-multiwalled carbon nanotube-alcohol dehydrogenase
Yu-Chen Tsai1, Jing-Dae Huang, Chian-Cheng Chiu
1Department of Chemical Engineering, National Chung Hsing University, 250, Kuo Kuang Road, Taichung 402, Taiwan. yctsai@dragon.nchu.edu.tw
Abstract:
A novel amperometric ethanol biosensor was constructed using alcohol dehydrogenase (ADH) physically immobilized within poly(vinyl alcohol)-multiwalled carbon nanotube (PVA-MWCNT) composite obtained by a freezing-thawing process. It comprises a MWCNT conduit, a PVA binder, and an ADH function. The measurement of ethanol is based on the signal produced by beta-nicotinamide adenine dinucleotide (NADH), the product of the enzymatic reaction. The homogeneity of the resulting biocomposite film was characterized by atomic force microscopy (AFM). The performance of the PVA-MWCNT-ADH biocomposite modified glassy carbon electrode was evaluated using cyclic voltammetry and amperometry in the presence of NADH and in the presence of ethanol. The ethanol content in standard solutions was determined and a sensitivity of 196 nA mM(-1), a linear range up to 1.5mM, and a response time of about 8s were obtained. These characteristics allowed its application for direct detection of ethanol in alcoholic beverages: beer, red wine, and spirit.
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