Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

A single-molecule barcoding system using nanoslits for DNA analysis.

Kyubong Jo¹, Dalia M Dhingra, Theo Odijk

¹Laboratory for Molecular and Computational Genomics, University of Wisconsin, 425 Henry Mall, Madison, WI 53706, USA.

Proceedings of the National Academy of Sciences of the United States of America

|February 14, 2007

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effect of ensemble averaging on Green-Kubo estimation of short-time stress relaxation modulus in all-atom molecular dynamics simulations of an unentangled polymer melt.

The Journal of chemical physics·2026

Same author

Origins of Enhanced Ion Transport in Nanostructured Anion-Conducting Polyelectrolytes.

Journal of the American Chemical Society·2026

Same author

Impact of Small-Alkane Solvents on Polyolefin Hydrogenolysis over a Ruthenium Catalyst.

Industrial & engineering chemistry research·2026

Same author

Lipid Composition Determines Hybrid Nanoparticle Selectivity: Beyond Membrane Mimicry in Cancer Targeting.

Nano letters·2026

Same author

Characterizing Defect Dynamics in Silicon Carbide Using Symmetry-Adapted Collective Variables and Machine Learning Interatomic Potentials.

Journal of chemical theory and computation·2026

Same author

Controlled propagation of soliton bullets in an engineered strain field.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026

See all related articles

Researchers developed new nanoscale devices to elongate DNA molecules for genome analysis. This breakthrough integrates micro/nanoscale features and enzymatic labeling for scalable, single-molecule DNA sequencing.

Area of Science:

Biotechnology
Genomics
Nanotechnology

Background:

Single-molecule DNA analysis requires precise control over large DNA molecules.
Existing nanoscale devices face challenges in fabrication, sample loading, labeling, and detection.

Purpose of the Study:

To develop integrated strategies for scalable genome analysis using molecular confinement.
To create disposable micro/nanoscale devices for DNA elongation and sequencing.

Main Methods:

Controlled buffer conditions to alter DNA stiffness and induce elongation within micro/nanoscale devices.
Development of analytical calculations to describe DNA elongation.
Enzymatic labeling of specific DNA sequences within nanoslit devices.
Fluorescence resonance energy transfer (FRET) for imaging elongated DNA molecules.

Related Experiment Videos

Main Results:

Demonstrated significant elongation of DNA molecules using disposable devices with micro/nanoscale features.
Validated analytical models describing DNA molecule elongation.
Successfully tagged specific sequences on elongated DNA molecules using enzymatic methods.
Enabled high-resolution imaging of labeled, elongated DNA via FRET.

Conclusions:

Integrated micro/nanoscale devices and enzymatic labeling offer a scalable approach for genome analysis.
Controlled molecular confinement and DNA manipulation are key for advancing single-molecule sequencing.
This work lays the foundation for robust, high-throughput DNA sequencing platforms.